11930   Smarcd2     Status: ES cell colonies screened / QC positives

GeneSmarcd2
Chromosome11
Deletion Start106,272,705
Deletion End106,264,057
Deletion Size8,649
Genome Build38
MGI IDMGI:1933621
NCBI Gene ID83796
BAC ID261A23
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF106,272,001106,272,023GGAACTGGCAGAGCGTTAGTATG
TUP106,271,977106,271,999CTGAGATGGCTCCCAGCTGCTCA
TUR106,271,957106,271,975GGCTTCCTTCCTCCTCAAC
TDF106,264,573106,264,593GAGCTGGAGCACATTTGACAC
TDP106,264,539106,264,566CCCTTCTCCAGATCCATGAGACCATTGA
TDR106,264,513106,264,535TCTGGGTCTTTAGCTGGTTGATG

PCR Primers

PrimerSequence
SUTAACCGCCTCTGGGTAACTG
SDTAAACATGCAAACCCAGCAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR67 bp++-
TDFTDR81 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11930A-E1VGB6C57BL/6NTac
11930A-G1VGB6C57BL/6NTac
11930A-B2VGB6C57BL/6NTac
11930A-F4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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