| Gene | Ltc4s |
| Chromosome | 11 |
| Deletion Start | 50,238,388 |
| Deletion End | 50,236,508 |
| Deletion Size | 1,881 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,237,608 | 50,237,629 | GCATCACCCTAGTCAATCAACC |
| TUP | 50,237,575 | 50,237,601 | CCTACAGGGCCTCTCCATTGAACACCA |
| TUR | 50,237,547 | 50,237,565 | AATGCCCTTCTCCCACCTG |
| TDF | 50,237,300 | 50,237,318 | GGTTCGGGTGTCAGCCAAG |
| TDP | 50,237,280 | 50,237,298 | TGAACCACGCGCTCACCCA |
| TDR | 50,237,220 | 50,237,239 | CAGAGTGTGGCGAGGAACAG |
PCR Primers
| Primer | Sequence |
| SU | ACTGGAAGGAAGTGGTCAAG |
| SD | TGTCTAGAAGACAGTACAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11931A-A6 | VGB6 | C57BL/6NTac | |
| 11931A-B3 | VGB6 | C57BL/6NTac | |
| 11931A-C6 | VGB6 | C57BL/6NTac | |
| 11931A-H1 | VGB6 | C57BL/6NTac | |
| 11931A-G3 | VGB6 | C57BL/6NTac | |
| 11931A-H7 | VGB6 | C57BL/6NTac | |
