| Gene | Rbm3 |
| Chromosome | X |
| Deletion Start | 8,141,595 |
| Deletion End | 8,139,804 |
| Deletion Size | 1,792 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,144,615 | 8,144,636 | TGGCTTCATCACCTTCACAAAC |
| TUP | 8,144,589 | 8,144,614 | CCAGAGCATGCCTCAGATGCGATGAG |
| TUR | 8,144,565 | 8,144,586 | GGCCTCACCTCTCCATTCATAG |
| TDF | 8,144,085 | 8,144,104 | TTCTGGTCAGCGGAGCTTGG |
| TDP | 8,144,059 | 8,144,082 | TGGACGAGGCACCCAGAATTTGGT |
| TDR | 8,144,006 | 8,144,025 | GAATGGGCACAGGCAGGTAG |
PCR Primers
| Primer | Sequence |
| SU | CATGCGCGATAGGAGTCATC |
| SD | TTCAACTAGCTCTGGCATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11935A-A12 | VGB6 | C57BL/6NTac | |
| 11935A-C6 | VGB6 | C57BL/6NTac | |
| 11935A-G6 | VGB6 | C57BL/6NTac | |
