| Gene | Wrb |
| Chromosome | 16 |
| Deletion Start | 96,145,480 |
| Deletion End | 96,155,998 |
| Deletion Size | 10,519 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,145,514 | 96,145,533 | TGGTGTTGAGCTTCGTGTTC |
| TUP | 96,145,539 | 96,145,561 | TAACTTGCTGCGCATCCTCCTGC |
| TUR | 96,145,569 | 96,145,591 | GGCAGCTACTTACAAACGAGGAC |
| TDF | 96,155,712 | 96,155,732 | TTGGTAGACCAGGGACTTCTC |
| TDP | 96,155,735 | 96,155,759 | TCAGGAGATGCATAGTGCAGCCCTG |
| TDR | 96,155,815 | 96,155,838 | GCCTTAGTTACCATCCACCTACAG |
PCR Primers
| Primer | Sequence |
| SU | TCGGTTACTACAAGCAATCAC |
| SD | TAGTAGTGGTACGAAAGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 127 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11937A-C3 | VGB6 | C57BL/6NTac | |
| 11937A-F3 | VGB6 | C57BL/6NTac | |
| 11937A-G7 | VGB6 | C57BL/6NTac | |
| 11937A-G12 | VGB6 | C57BL/6NTac | |
