Gene | Med28 |
Chromosome | 5 |
Deletion Start | 45,520,329 |
Deletion End | 45,525,352 |
Deletion Size | 5,024 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 45,520,451 | 45,520,472 | CACTTTGGTGGACGAGTTGGAG |
TUP | 45,520,474 | 45,520,500 | CATCCTTCGAGGTAAGCCGAGATGTGG |
TUR | 45,520,503 | 45,520,520 | TGAGGGCTGCACAGAGAC |
TDF | 45,525,245 | 45,525,266 | GGACATCAATGTGCAGCACAAG |
TDP | 45,525,270 | 45,525,290 | CCGGCTGACATGCCTCAGGGA |
TDR | 45,525,323 | 45,525,342 | GCTTCAGAGGTGCAGGAATG |
PCR Primers
Primer | Sequence |
SU | GAAAAGACTCGGCTAAGGTC |
SD | GATGAAGACACAGAATGTTGTC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 70 bp | + | + | - |
TDF | TDR | 98 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
11940A-A8 | VGB6 | C57BL/6NTac | |
11940A-B3 | VGB6 | C57BL/6NTac | |
11940A-F12 | VGB6 | C57BL/6NTac | |
11940A-C12 | VGB6 | C57BL/6NTac | |