| Gene | D4Wsu53e |
| Chromosome | 4 |
| Deletion Start | 134,923,930 |
| Deletion End | 134,927,101 |
| Deletion Size | 3,172 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,924,441 | 134,924,459 | CGCGCAGCAGGAGTAGAAC |
| TUP | 134,924,462 | 134,924,488 | CATTTCGCCTGAGTGAGAAAGGTGCGT |
| TUR | 134,924,497 | 134,924,517 | ACCAAGGGCACAAACCTCAAC |
| TDF | 134,926,163 | 134,926,186 | GGCTGGGATGTAACTCAGTTGTAG |
| TDP | 134,926,187 | 134,926,216 | AGAACTTTGTCAGGGTGCATAGTACCTGGG |
| TDR | 134,926,217 | 134,926,236 | ACACAGTGCTGGGAGCTAAC |
PCR Primers
| Primer | Sequence |
| SU | GCCACTAAGCTCAGTCCCAG |
| SD | CAACCTCCCTAATGCAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11942A-A7 | VGB6 | C57BL/6NTac | |
| 11942A-A10 | VGB6 | C57BL/6NTac | |
| 11942A-G12 | VGB6 | C57BL/6NTac | |
| 11942A-E3 | VGB6 | C57BL/6NTac | |
| 11942A-G7 | VGB6 | C57BL/6NTac | |
