| Gene | Slc6a5 |
| Chromosome | 7 |
| Deletion Start | 49,911,725 |
| Deletion End | 49,938,396 |
| Deletion Size | 26,672 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,912,163 | 49,912,183 | ATGAGCCAGAGCACAGTAGTG |
| TUP | 49,912,184 | 49,912,205 | TTGGGAACCGATGGCATCGCGT |
| TUR | 49,912,244 | 49,912,263 | GCCGCTTGTGACCTGCTTAC |
| TDF | 49,938,303 | 49,938,320 | AAGGATGCGGCCACTCAG |
| TDP | 49,938,323 | 49,938,345 | TTTCTTCTCCCTGTCTGCGGCCT |
| TDR | 49,938,374 | 49,938,396 | AGCAGTTGTTGTGGAATTTGTTG |
PCR Primers
| Primer | Sequence |
| SU | AACCCACTCATTCTTCGGTC |
| SD | ACATGACTACTCCATGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11954C-B1 | VGB6 | C57BL/6NTac | |
| 11954D-E2 | VGB6 | C57BL/6NTac | |
