| Gene | Surf1 |
| Chromosome | 2 |
| Deletion Start | 26,916,328 |
| Deletion End | 26,913,343 |
| Deletion Size | 2,986 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,915,826 | 26,915,847 | TTGCCACCTTTGCCATACATTC |
| TUP | 26,915,792 | 26,915,818 | TATAATCGGGCCTCTCGAGGAATTGGC |
| TUR | 26,915,762 | 26,915,785 | CAGAAGCCCTAGAACAAACAGAAC |
| TDF | 26,913,613 | 26,913,632 | GGAGGACAGACGAGAGTGAC |
| TDP | 26,913,587 | 26,913,610 | TGCGCAATGAGCACATGCAGTACA |
| TDR | 26,913,558 | 26,913,576 | AGCAGGCAGCAGGACTAAC |
PCR Primers
| Primer | Sequence |
| SU | TTTCCAGGCACAATACTCCC |
| SD | CTTCCTCATTGCCTTTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11956A-H9 | VGB6 | C57BL/6NTac | |
| 11956A-B8 | VGB6 | C57BL/6NTac | |
| 11956A-D8 | VGB6 | C57BL/6NTac | |
| 11956A-F9 | VGB6 | C57BL/6NTac | |
| 11956A-E2 | VGB6 | C57BL/6NTac | |
