11958   Atp5b     Status: ES cell colonies screened / QC positives

GeneAtp5b
Chromosome10
Deletion Start128,083,330
Deletion End128,090,130
Deletion Size6,801
Genome Build38
MGI IDMGI:107801
NCBI Gene ID11947
BAC ID291K21
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF128,083,959128,083,977AGTCATCGGCGCTGTGGTG
TUP128,083,979128,084,006ACGTCCAGTTCGATGAGGGATTACCACC
TUR128,084,029128,084,047GTCTGCTGTCCCTGCCTTG
TDF128,089,593128,089,614GAGTACACGATCCCATGAGGAG
TDP128,089,617128,089,643AGATCCTCCCAGAACAGTCAGTGGAGG
TDR128,089,694128,089,715GGCTTGAGTGGTGGTTTAACAC

PCR Primers

PrimerSequence
SUTAGGTTTCGAGGCTTAGAGG
SDCACTTTAGTGATAGTTGGAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR89 bp++-
TDFTDR123 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11958A-G4VGB6C57BL/6NTac
11958B-F4VGB6C57BL/6NTac
11958B-D2VGB6C57BL/6NTac
11958B-A10VGB6C57BL/6NTac
11958B-B4VGB6C57BL/6NTac
11958B-G6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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