| Gene | Wbscr22 |
| Chromosome | 5 |
| Deletion Start | 135,064,058 |
| Deletion End | 135,053,348 |
| Deletion Size | 10,711 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 135,063,170 | 135,063,193 | CAGACCCTCTTGTCTCTACCTTAC |
| TUP | 135,063,136 | 135,063,158 | TTGCAGACATGGTCCCGCACGTA |
| TUR | 135,063,109 | 135,063,132 | CCCACAGAAGAGTGTTCCATACAG |
| TDF | 135,053,728 | 135,053,747 | TTGGTTCTGTTGCTGAGGAG |
| TDP | 135,053,700 | 135,053,727 | TCCAGAGTGGAAATCACAGAAGGGAAGA |
| TDR | 135,053,654 | 135,053,675 | GCGAGAGGATGGCTAAGATGTG |
PCR Primers
| Primer | Sequence |
| SU | GAAGCTCTGCCGGTAGTACG |
| SD | GGCCAGGGACACCAGTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11959A-A4 | VGB6 | C57BL/6NTac | |
| 11959A-G1 | VGB6 | C57BL/6NTac | |
| 11959A-D2 | VGB6 | C57BL/6NTac | |
| 11959A-F4 | VGB6 | C57BL/6NTac | |
| 11959A-G8 | VGB6 | C57BL/6NTac | |
