| Gene | Scnm1-ps |
| Chromosome | 3 |
| Deletion Start | 95,133,892 |
| Deletion End | 95,129,714 |
| Deletion Size | 4,179 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,133,647 | 95,133,671 | CATCCGTCCCTCTTACTCATTTCTC |
| TUP | 95,133,622 | 95,133,645 | TATCCTGCTCCCTGCAGAAACGGA |
| TUR | 95,133,572 | 95,133,591 | CATCAGTGCCTCGTCCTCAG |
| TDF | 95,130,351 | 95,130,370 | TTCACTGCCCAGACCAGAAG |
| TDP | 95,130,326 | 95,130,348 | CCTGCTCCCTCTGTCTCCAGTCC |
| TDR | 95,130,282 | 95,130,303 | TGATCTCTGCACTCTGGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGGTCCTGTGTCCAGGTGAG |
| SD | CTCTCAGCAGGGTACTTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11960A-G8 | VGB6 | C57BL/6NTac | |
| 11960A-C5 | VGB6 | C57BL/6NTac | |
| 11960A-A12 | VGB6 | C57BL/6NTac | |
| 11960A-C2 | VGB6 | C57BL/6NTac | |
| 11960A-F10 | VGB6 | C57BL/6NTac | |
