| Gene | Pigo |
| Chromosome | 4 |
| Deletion Start | 43,025,560 |
| Deletion End | 43,018,384 |
| Deletion Size | 7,177 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,024,653 | 43,024,674 | CGTCTAAAGGCTCTCACCACTG |
| TUP | 43,024,626 | 43,024,650 | TCGCTGCCGACTTTCATTGATGCTG |
| TUR | 43,024,604 | 43,024,622 | GCATGGCTGGCAAAGTTAC |
| TDF | 43,018,141 | 43,018,162 | GGTGTTTGCTCCCAAGTAAGTG |
| TDP | 43,018,113 | 43,018,136 | CCCGTGAGAGGGAACTCTTCGTGC |
| TDR | 43,018,086 | 43,018,107 | AAAGAGGCCAACCATCATCAAC |
PCR Primers
| Primer | Sequence |
| SU | GTTGGATATGCACAGGCTCC |
| SD | CACCTCACAGATCTTAGCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11961A-B12 | VGB6 | C57BL/6NTac | |
| 11961A-F5 | VGB6 | C57BL/6NTac | |
