| Gene | Pnlip |
| Chromosome | 19 |
| Deletion Start | 58,670,542 |
| Deletion End | 58,681,736 |
| Deletion Size | 11,195 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,670,648 | 58,670,670 | CCAGGTTGAGGATGAGGACTTAG |
| TUP | 58,670,673 | 58,670,698 | AAGAGCAATGCAGGCTTAAGGAAGCC |
| TUR | 58,670,738 | 58,670,756 | CACTGCAAGCTGCAAACAC |
| TDF | 58,680,839 | 58,680,861 | GATCAACCCAACTCTACCCAAAG |
| TDP | 58,680,862 | 58,680,887 | TGGGAGCATCAAGGATCACAGTGGAA |
| TDR | 58,680,889 | 58,680,910 | ACTCACACTCTGCCATCATTTC |
PCR Primers
| Primer | Sequence |
| SU | TAGAAGTACGTCCTTGGAGC |
| SD | TGACCAGAGAGATAACAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11962A-E4 | VGB6 | C57BL/6NTac | |
| 11962B-C12 | VGB6 | C57BL/6NTac | |
| 11962C-B10 | VGB6 | C57BL/6NTac | |
