| Gene | Lsm3 |
| Chromosome | 6 |
| Deletion Start | 91,516,061 |
| Deletion End | 91,522,328 |
| Deletion Size | 6,268 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,516,459 | 91,516,483 | GATGGAGAAACTGCTAGAGATGGAG |
| TUP | 91,516,494 | 91,516,523 | ACAAGCAAAGATCTGGTACACAGGCCAACA |
| TUR | 91,516,538 | 91,516,561 | AATAGGGAAGGGTAGGTGCAGAGG |
| TDF | 91,522,169 | 91,522,187 | CTGCGGTCACCCAGGAAAC |
| TDP | 91,522,188 | 91,522,214 | AGGCACTCAGACTGCTTACCCTGATCC |
| TDR | 91,522,278 | 91,522,298 | CGACTAGAACGACGCCATCTC |
PCR Primers
| Primer | Sequence |
| SU | TGTTGTCCTCGGTTTTCTGG |
| SD | AGACACACTGAACACACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11965A-F11 | VGB6 | C57BL/6NTac | |
| 11965A-B12 | VGB6 | C57BL/6NTac | |
