| Gene | Tsc22d4 |
| Chromosome | 5 |
| Deletion Start | 137,747,119 |
| Deletion End | 137,759,284 |
| Deletion Size | 12,166 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,747,884 | 137,747,903 | GGGCGGGACCTACTGTACTC |
| TUP | 137,747,907 | 137,747,932 | CGGCTGACCTATATCCTGGGCCTCCT |
| TUR | 137,747,936 | 137,747,954 | CGGCAGGACGGAAACTGAG |
| TDF | 137,759,059 | 137,759,078 | GGGTCCAGGACTTGGTGAAG |
| TDP | 137,759,081 | 137,759,102 | CCACCTCATGTTTGCCGTGCGA |
| TDR | 137,759,150 | 137,759,168 | CTGCTCCAATGCAGCGTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTCAGCTTTGGGGTTTCAG |
| SD | CTCCCCAGAACACTGATACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11968B-B5 | VGB6 | C57BL/6NTac | |
| 11968B-C9 | VGB6 | C57BL/6NTac | |
| 11968B-E6 | VGB6 | C57BL/6NTac | |
| 11968B-G2 | VGB6 | C57BL/6NTac | |
| 11968C-E5 | VGB6 | C57BL/6NTac | |
| 11968C-E6 | VGB6 | C57BL/6NTac | |
