| Gene | Pkp2 |
| Chromosome | 16 |
| Deletion Start | 16,213,376 |
| Deletion End | 16,240,704 |
| Deletion Size | 27,329 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,214,015 | 16,214,034 | CAAGTGCTCCACTCCGTATC |
| TUP | 16,214,035 | 16,214,062 | TGCGACTAAACAAATTGAGCAAGTCGGC |
| TUR | 16,214,083 | 16,214,103 | CTTATCCTCCGCAGGGTGTTC |
| TDF | 16,240,526 | 16,240,550 | GCTTCTACAACTGCTCAAACTTCAG |
| TDP | 16,240,551 | 16,240,577 | AACGAAGATGTTCAACGGGCTGCTTGT |
| TDR | 16,240,630 | 16,240,651 | CGAGGCACTCCATTCAGTTCAG |
PCR Primers
| Primer | Sequence |
| SU | TGCTACTCACAGCAGAAGTTC |
| SD | CGCATGCTCAAACCAAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11973C-B11 | VGB6 | C57BL/6NTac | |
| 11973C-C10 | VGB6 | C57BL/6NTac | |
| 11973B-D3 | VGB6 | C57BL/6NTac | |
| 11973B-E2 | VGB6 | C57BL/6NTac | |
| 11973C-D11 | VGB6 | C57BL/6NTac | |
| 11973C-F5 | VGB6 | C57BL/6NTac | |
