| Gene | Rimklb |
| Chromosome | 6 |
| Deletion Start | 122,479,908 |
| Deletion End | 122,466,292 |
| Deletion Size | 13,617 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,472,567 | 122,472,588 | GGCTGTGGTGATGGATGAGATG |
| TUP | 122,472,542 | 122,472,565 | TGCTGACGGTCGAGCAAGGAAATC |
| TUR | 122,472,486 | 122,472,507 | GCTCTGTGAGGCAGTCCTTATG |
| TDF | 122,459,155 | 122,459,176 | CGAGTCATTGTTGTGGGAGGTC |
| TDP | 122,459,129 | 122,459,153 | TGTAGTTGGCACCATGCTACGCTGT |
| TDR | 122,459,096 | 122,459,118 | GGAACAGTTGCTTTGCATCCTTC |
PCR Primers
| Primer | Sequence |
| SU | CTAAGCAGGGTTCCTCCAG |
| SD | GCTTTTAAGGTGACAAAGGGA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11974A-A8 | VGB6 | C57BL/6NTac | |
| 11974A-H1 | VGB6 | C57BL/6NTac | |
