| Gene | Aldoc |
| Chromosome | 11 |
| Deletion Start | 78,324,405 |
| Deletion End | 78,326,357 |
| Deletion Size | 1,953 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,325,273 | 78,325,294 | GGCTTTCTCCCATCCTATCTTC |
| TUP | 78,325,296 | 78,325,322 | ACTTCTTCCAGTTAACCCTGCTCTTGC |
| TUR | 78,325,354 | 78,325,374 | CTCCGTCAGGCAGAATCTCAG |
| TDF | 78,326,197 | 78,326,215 | GCAGCGTGCATTCCACATC |
| TDP | 78,326,216 | 78,326,238 | CGTGGTCTGGCTCAGTCTGCTCA |
| TDR | 78,326,259 | 78,326,278 | GCTGCAAGCCCGTTCATCTG |
PCR Primers
| Primer | Sequence |
| SU | ACCTGGCAGCAGTTTCCAG |
| SD | CTATACCATCAAGGGTAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11975A-B1 | VGB6 | C57BL/6NTac | |
| 11975A-C6 | VGB6 | C57BL/6NTac | |
| 11975A-D3 | VGB6 | C57BL/6NTac | |
| 11975A-D4 | VGB6 | C57BL/6NTac | |
| 11975A-D5 | VGB6 | C57BL/6NTac | |
| 11975A-E5 | VGB6 | C57BL/6NTac | |
