| Gene | Agxt |
| Chromosome | 1 |
| Deletion Start | 93,135,297 |
| Deletion End | 93,145,345 |
| Deletion Size | 10,049 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,135,671 | 93,135,688 | GGTTGTCAGCGGTTCAGG |
| TUP | 93,135,691 | 93,135,713 | ACTGCGCCATGGAGACTGCCCTG |
| TUR | 93,135,744 | 93,135,764 | GATGCCATTGGTTCCGGTTAG |
| TDF | 93,145,021 | 93,145,038 | CCAACAGCGGCTGACATC |
| TDP | 93,145,041 | 93,145,067 | CATGTTCCATGCCTACCAGGTGCTACG |
| TDR | 93,145,099 | 93,145,118 | GCCACACGGTCCACATTCTC |
PCR Primers
| Primer | Sequence |
| SU | CAATACATCGGACCTACAAGC |
| SD | CAGTATGTTTGGTATGTGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11976A-A6 | VGB6 | C57BL/6NTac | |
| 11976A-A8 | VGB6 | C57BL/6NTac | |
