| Gene | Coro1a |
| Chromosome | 7 |
| Deletion Start | 126,704,699 |
| Deletion End | 126,701,361 |
| Deletion Size | 3,339 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,703,140 | 126,703,159 | GGTTCGCTCCAGCAAATTCC |
| TUP | 126,703,116 | 126,703,137 | CACGTGTTTGGACAGCCAGCCA |
| TUR | 126,703,082 | 126,703,102 | GAGACGCGCACATCCTCATAG |
| TDF | 126,700,790 | 126,700,811 | CAAGGAGTCTCAGCGTGGTATG |
| TDP | 126,700,764 | 126,700,787 | CTACATGCCCAAACGTGGCCTGGA |
| TDR | 126,700,729 | 126,700,748 | GAGTCAGTCACCTGGCAATC |
PCR Primers
| Primer | Sequence |
| SU | TGTCTTAGTCAAGAACCAGAC |
| SD | AGGGATTTGGATGACTCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11977B-E7 | VGB6 | C57BL/6NTac | |
| 11977C-A9 | VGB6 | C57BL/6NTac | |
| 11977C-B6 | VGB6 | C57BL/6NTac | |
