| Gene | Cyp27b1 |
| Chromosome | 10 |
| Deletion Start | 127,049,050 |
| Deletion End | 127,052,152 |
| Deletion Size | 3,103 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,049,155 | 127,049,174 | CAGCTCCTGCGACAAGAAAG |
| TUP | 127,049,176 | 127,049,202 | CACTGTCCAGAGCGCTGTAGTTTCTCA |
| TUR | 127,049,242 | 127,049,259 | TCACGCCGTTAGCAATCC |
| TDF | 127,051,619 | 127,051,641 | ACCCATTTGCATCTCTTCCCTTC |
| TDP | 127,051,645 | 127,051,667 | TTTGGCAAACGGAGCTGCATCGG |
| TDR | 127,051,674 | 127,051,694 | TGTAGCTCAAGCTCTGCCAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTTGAGGTACAGTTAGGAG |
| SD | ATCAACGAACACTACAGGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11980A-A7 | VGB6 | C57BL/6NTac | |
| 11980A-A10 | VGB6 | C57BL/6NTac | |
| 11980A-B1 | VGB6 | C57BL/6NTac | |
| 11980A-B6 | VGB6 | C57BL/6NTac | |
| 11980A-B10 | VGB6 | C57BL/6NTac | |
| 11980A-C11 | VGB6 | C57BL/6NTac | |
| 11980A-D11 | VGB6 | C57BL/6NTac | |
| 11980A-D7 | VGB6 | C57BL/6NTac | |
