| Gene | Lhb |
| Chromosome | 7 |
| Deletion Start | 45,420,956 |
| Deletion End | 45,421,806 |
| Deletion Size | 851 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,421,288 | 45,421,305 | GGCCTGTCAACGCAACTC |
| TUP | 45,421,312 | 45,421,338 | CAGAGAATGAGTTCTGCCCAGTCTGCA |
| TUR | 45,421,343 | 45,421,363 | GCACAGATGCTGGTGGTGAAG |
| TDF | 45,421,564 | 45,421,582 | CTGTGCCTCAGCCAGTGTG |
| TDP | 45,421,583 | 45,421,604 | CACCTACCGGGAGCTGCGCTTC |
| TDR | 45,421,646 | 45,421,668 | GGGCTACAGGAAAGGAGACTATG |
PCR Primers
| Primer | Sequence |
| SU | TTGAAAGCAAATTTGGAGGC |
| SD | GCTCTGATCTGAGTCCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11981A-F10 | VGB6 | C57BL/6NTac | |
| 11981B-B7 | VGB6 | C57BL/6NTac | |
| 11981B-C4 | VGB6 | C57BL/6NTac | |
| 11981B-C5 | VGB6 | C57BL/6NTac | |
| 11981B-G6 | VGB6 | C57BL/6NTac | |
