| Gene | Cln5 |
| Chromosome | 14 |
| Deletion Start | 103,070,349 |
| Deletion End | 103,076,339 |
| Deletion Size | 5,991 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,070,854 | 103,070,876 | GGCTAGTCAGGATCACCTTGTTG |
| TUP | 103,070,878 | 103,070,904 | TCCACTTCAACCTGGAGGGTAGGTAGG |
| TUR | 103,070,921 | 103,070,941 | AAGTCAAAGACTGCGATCACC |
| TDF | 103,075,374 | 103,075,394 | TGCATGTCGCTTAGCTGTGAC |
| TDP | 103,075,395 | 103,075,415 | AGGGCAGCAGCTCCCTCAGCA |
| TDR | 103,075,428 | 103,075,448 | AGGGCAGATGGATGCTTGGTG |
PCR Primers
| Primer | Sequence |
| SU | CTAGAGGCATAGCACACATC |
| SD | ACGAAACAGCACTACTGTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11986A-D9 | VGB6 | C57BL/6NTac | |
| 11986A-G3 | VGB6 | C57BL/6NTac | |
