| Gene | S100a4 |
| Chromosome | 3 |
| Deletion Start | 90,605,015 |
| Deletion End | 90,605,947 |
| Deletion Size | 933 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,605,030 | 90,605,052 | GAGGCCCTGGATGTAATTGTGTC |
| TUP | 90,605,053 | 90,605,082 | CACCTTCCACAAATACTCAGGCAAAGAGGG |
| TUR | 90,605,120 | 90,605,139 | GGCAGCTCCCTGGTCAGTAG |
| TDF | 90,605,755 | 90,605,775 | GCTGCATTCCAGAAGGTGATG |
| TDP | 90,605,776 | 90,605,800 | AGCAACTTGGACAGCAACAGGGACA |
| TDR | 90,605,823 | 90,605,842 | TGCAGGACAGGAAGACACAG |
PCR Primers
| Primer | Sequence |
| SU | GTTGAGGCCAGGACTGAGAG |
| SD | TTAGTGTTCCCGGAGTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11987A-A1 | VGB6 | C57BL/6NTac | |
| 11987A-A5 | VGB6 | C57BL/6NTac | |
| 11987A-C5 | VGB6 | C57BL/6NTac | |
| 11987A-C9 | VGB6 | C57BL/6NTac | |
| 11987A-F6 | VGB6 | C57BL/6NTac | |
| 11987A-D12 | VGB6 | C57BL/6NTac | |
