| Gene | Oat |
| Chromosome | 7 |
| Deletion Start | 132,575,655 |
| Deletion End | 132,563,724 |
| Deletion Size | 11,932 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,569,760 | 132,569,778 | GGCGACTAAGGCTCCTGTG |
| TUP | 132,569,736 | 132,569,759 | CCCAGCAGTCTTCCATGGGAGCCT |
| TUR | 132,569,708 | 132,569,728 | GCCCATTTCCTCTGCATTCAC |
| TDF | 132,558,298 | 132,558,316 | AAGGTGTGCCTGCGACTTC |
| TDP | 132,558,272 | 132,558,294 | ATAACGGGCTTCTGGCCAAGCCA |
| TDR | 132,558,190 | 132,558,211 | TGTTGATGATCTCCACGGACTC |
PCR Primers
| Primer | Sequence |
| SU | AGGGCCATTTGAACCCAGAG |
| SD | AAGGAAACTGTAAAGACGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 127 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11989A-B6 | VGB6 | C57BL/6NTac | |
| 11989A-F2 | VGB6 | C57BL/6NTac | |
