| Gene | Minpp1 |
| Chromosome | 19 |
| Deletion Start | 32,485,944 |
| Deletion End | 32,514,375 |
| Deletion Size | 28,432 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,486,745 | 32,486,762 | GCGCAAGAGTGCAGTAGG |
| TUP | 32,486,765 | 32,486,788 | ACGCTGGAGACCCAGCTAGAAGGA |
| TUR | 32,486,818 | 32,486,839 | CGCGATTTGGGAATGTGGAATG |
| TDF | 32,514,253 | 32,514,273 | AGACCCGTCGGGTTGTATGAG |
| TDP | 32,514,275 | 32,514,298 | AGCTCAAGACCCACTACCGGGACA |
| TDR | 32,514,339 | 32,514,358 | CGGACGTGATGTTCGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | CTTTGAATCGGTTCCTGAGG |
| SD | AAATCTCAGCTCTGCAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11994A-F3 | VGB6 | C57BL/6NTac | |
| 11994A-B2 | VGB6 | C57BL/6NTac | |
