| Gene | Aip |
| Chromosome | 19 |
| Deletion Start | 4,121,380 |
| Deletion End | 4,114,482 |
| Deletion Size | 6,899 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,121,363 | 4,121,383 | ATACAGGAGGGCCGAGGAGAG |
| TUP | 4,121,340 | 4,121,362 | TTGCCGGACTTTCAGGATGGCAC |
| TUR | 4,121,294 | 4,121,315 | CAGAAGAGACGCTGGGAAAGTG |
| TDF | 4,115,118 | 4,115,142 | GGTGGCTCAGGAGTATTATGAAGTG |
| TDP | 4,115,093 | 4,115,116 | TGGATCACTGCTCCTCCATCCTCA |
| TDR | 4,115,035 | 4,115,053 | GGATGGGAAGAGGCCAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGCCAACTAAGCACAACTGG |
| SD | CTAGGCTGGTATTGGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11995A-A1 | VGB6 | C57BL/6NTac | |
| 11995A-B6 | VGB6 | C57BL/6NTac | |
| 11995A-C3 | VGB6 | C57BL/6NTac | |
| 11995A-E1 | VGB6 | C57BL/6NTac | |
| 11995A-E3 | VGB6 | C57BL/6NTac | |
| 11995A-E6 | VGB6 | C57BL/6NTac | |
