| Gene | Thap7 |
| Chromosome | 16 |
| Deletion Start | 17,530,881 |
| Deletion End | 17,528,180 |
| Deletion Size | 2,702 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,530,198 | 17,530,217 | GCCAGCTAACACCAGGACAG |
| TUP | 17,530,168 | 17,530,193 | TGAGGCATCAGAGCAGTGGGTCAAAC |
| TUR | 17,530,140 | 17,530,158 | GGACTCCACCAGGGCAAAG |
| TDF | 17,528,260 | 17,528,278 | ACGGGAGAAGCGAGCACAG |
| TDP | 17,528,232 | 17,528,257 | AGATGCCCGCCAGACTCTGAAGGACC |
| TDR | 17,528,205 | 17,528,226 | TCAACTGCATAGCAAAGTCCTG |
PCR Primers
| Primer | Sequence |
| SU | AATGGCAAGACCGTGAGTAC |
| SD | AGCTAACCTCTGAGGAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11998A-A8 | VGB6 | C57BL/6NTac | |
| 11998A-A11 | VGB6 | C57BL/6NTac | |
| 11998A-B7 | VGB6 | C57BL/6NTac | |
| 11998A-B9 | VGB6 | C57BL/6NTac | |
| 11998A-C8 | VGB6 | C57BL/6NTac | |
| 11998A-D4 | VGB6 | C57BL/6NTac | |
| 11998A-C11 | VGB6 | C57BL/6NTac | |
