| Gene | Fyttd1 |
| Chromosome | 16 |
| Deletion Start | 32,877,897 |
| Deletion End | 32,905,678 |
| Deletion Size | 27,782 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,878,219 | 32,878,240 | TCCGTAGCTGCTGTCTTCTTTC |
| TUP | 32,878,241 | 32,878,262 | CCCGCAGGACGGAGTCTCTTGG |
| TUR | 32,878,337 | 32,878,359 | CGGTTGCTGGCTTTAGTCAGTTG |
| TDF | 32,905,564 | 32,905,585 | ACTGAAAGAGCAAAGAGCCAAC |
| TDP | 32,905,587 | 32,905,611 | TCACGTTCAGCAAAGGAGGAAGCCG |
| TDR | 32,905,616 | 32,905,638 | ACACGGATCTATCCCACAGTCAC |
PCR Primers
| Primer | Sequence |
| SU | AGTCGTTCAGACTCGGTTTC |
| SD | GACAACACAGAGGTGTACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 141 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12001A-H12 | VGB6 | C57BL/6NTac | |
| 12001A-F4 | VGB6 | C57BL/6NTac | |
| 12001A-G12 | VGB6 | C57BL/6NTac | |
| 12001A-G5 | VGB6 | C57BL/6NTac | |
| 12001A-G7 | VGB6 | C57BL/6NTac | |
| 12001A-B1 | VGB6 | C57BL/6NTac | |
