| Gene | Slc25a11 |
| Chromosome | 11 |
| Deletion Start | 70,646,435 |
| Deletion End | 70,644,325 |
| Deletion Size | 2,111 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,646,231 | 70,646,250 | TCCCTTTCCTGCCTCACTTC |
| TUP | 70,646,202 | 70,646,226 | TGGGTGCTACAGTCTTTGTGCAGCC |
| TUR | 70,646,161 | 70,646,181 | CTTCACCACTCAACTGCATCC |
| TDF | 70,644,906 | 70,644,927 | GTCTAGGATGTGCTGCTGAAAG |
| TDP | 70,644,880 | 70,644,905 | TCGTCCGCTATGAGGGTTTCTTCAGC |
| TDR | 70,644,846 | 70,644,868 | GTCGGGCATAGTATGGTGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCTCGCGAGGTTTTCAAAC |
| SD | ACTAGAAATCCTTGGTGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12002A-C5 | VGB6 | C57BL/6NTac | |
| 12002A-D5 | VGB6 | C57BL/6NTac | |
| 12002A-F3 | VGB6 | C57BL/6NTac | |
| 12002A-F2 | VGB6 | C57BL/6NTac | |
| 12002A-E5 | VGB6 | C57BL/6NTac | |
| 12002A-C9 | VGB6 | C57BL/6NTac | |
