| Gene | Cks1b |
| Chromosome | 3 |
| Deletion Start | 89,417,897 |
| Deletion End | 89,415,542 |
| Deletion Size | 2,356 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,417,525 | 89,417,546 | AGCTGGTACGGTAGCTCACATC |
| TUP | 89,417,491 | 89,417,516 | CAGCATTCTGGAAGCTGAGGCTGCAG |
| TUR | 89,417,470 | 89,417,490 | GGGCTGGACCCTATGACAATC |
| TDF | 89,416,419 | 89,416,440 | GACACAGTCCTCACTCACATCC |
| TDP | 89,416,386 | 89,416,415 | TGTTTCTGTTACAGGCATGTCATGTTGCCC |
| TDR | 89,416,362 | 89,416,380 | CGGGACCAGCTTGGCTATG |
PCR Primers
| Primer | Sequence |
| SU | GTGAGAGCCAATGAGAATCC |
| SD | CCATCCTTGTAACAGCCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12007A-A2 | VGB6 | C57BL/6NTac | |
| 12007A-B4 | VGB6 | C57BL/6NTac | |
| 12007A-B5 | VGB6 | C57BL/6NTac | |
| 12007A-D10 | VGB6 | C57BL/6NTac | |
| 12007A-B3 | VGB6 | C57BL/6NTac | |
