| Gene | Atp5o |
| Chromosome | 16 |
| Deletion Start | 91,931,552 |
| Deletion End | 91,925,278 |
| Deletion Size | 6,275 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,930,852 | 91,930,870 | GGCTGCTCCAGTGACAGAC |
| TUP | 91,930,827 | 91,930,850 | CAGTGCCTGAAGCCTGGTGCTGTA |
| TUR | 91,930,770 | 91,930,794 | CAAGGAAACCTACTTGCTTAGGAAC |
| TDF | 91,925,522 | 91,925,541 | GGTGGGTCTGCATGATTGTG |
| TDP | 91,925,496 | 91,925,518 | TGTTAGTGCTGAGGCCCGGCTTA |
| TDR | 91,925,474 | 91,925,495 | GGTGGGAACTACTCGGTATGTG |
PCR Primers
| Primer | Sequence |
| SU | GATGTTGACGATGCATGTGC |
| SD | TATGACTGGTTAGGGACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12016A-B8 | VGB6 | C57BL/6NTac | |
| 12016A-C4 | VGB6 | C57BL/6NTac | |
