| Gene | Lysmd1 |
| Chromosome | 3 |
| Deletion Start | 95,134,820 |
| Deletion End | 95,138,541 |
| Deletion Size | 3,722 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,134,924 | 95,134,943 | GGAAAGACGCCTGGAGCATC |
| TUP | 95,134,945 | 95,134,968 | ATTGGAGCCTGGAGACACCCTGGC |
| TUR | 95,135,016 | 95,135,037 | CACCCACTTTCTCGGGACTATC |
| TDF | 95,138,224 | 95,138,243 | GCCAGTGTGCTGGGATGAAG |
| TDP | 95,138,244 | 95,138,272 | CCTTAGGAGCAAGTTCTTCAGAGGGTCGC |
| TDR | 95,138,292 | 95,138,311 | AGGCTGCCTCCCTTTGTTTC |
PCR Primers
| Primer | Sequence |
| SU | TAAGGAGCTCGTGGAAAGAG |
| SD | GCATAGAAGAGCCTTTGAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12021A-E10 | VGB6 | C57BL/6NTac | |
| 12021A-D10 | VGB6 | C57BL/6NTac | |
