| Gene | Mrpl17 |
| Chromosome | 7 |
| Deletion Start | 105,810,816 |
| Deletion End | 105,809,690 |
| Deletion Size | 1,127 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,810,590 | 105,810,611 | CTGGTGGGATGGTTGGATGAAG |
| TUP | 105,810,561 | 105,810,588 | TAAGACAGAAGAGTAAGGTGGGCGCCTC |
| TUR | 105,810,537 | 105,810,559 | TGGATGCAAGACGAGTCAAGAAC |
| TDF | 105,809,955 | 105,809,976 | CACAGAGACAGCAACCTTACTC |
| TDP | 105,809,928 | 105,809,951 | TAAACCAGCTGCTCCTGGGACTGC |
| TDR | 105,809,896 | 105,809,916 | GCTTGCGTCCTGGTTATGGTG |
PCR Primers
| Primer | Sequence |
| SU | TCCAGAAACGTCAACACGTC |
| SD | AGCATCTGGATGCCTCTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12026A-E4 | VGB6 | C57BL/6NTac | |
| 12026A-H10 | VGB6 | C57BL/6NTac | |
