| Gene | Hnrpdl |
| Chromosome | 5 |
| Deletion Start | 100,038,802 |
| Deletion End | 100,036,103 |
| Deletion Size | 2,700 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,038,617 | 100,038,638 | GTCTTTCCCCGAGTCGATTCTC |
| TUP | 100,038,593 | 100,038,614 | CGTGCCCGCTGGACCCCAGTAG |
| TUR | 100,038,555 | 100,038,577 | TCGAGTCACCCCATCTTACTTTC |
| TDF | 100,036,840 | 100,036,858 | AGACCTCCTTGGGCGAGTG |
| TDP | 100,036,812 | 100,036,837 | CGGTACACTGAAGCAGTGAGCTGCTA |
| TDR | 100,036,785 | 100,036,806 | GCTGTGTTTCCCTACAGGTCAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCGCTCCCGCTACTTTAG |
| SD | CAGAGATAGACGTGGTCAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12030A-A3 | VGB6 | C57BL/6NTac | |
| 12030A-C1 | VGB6 | C57BL/6NTac | |
| 12030A-E1 | VGB6 | C57BL/6NTac | |
| 12030A-D12 | VGB6 | C57BL/6NTac | |
| 12030A-G4 | VGB6 | C57BL/6NTac | |
