| Gene | Parp11 |
| Chromosome | 6 |
| Deletion Start | 127,470,708 |
| Deletion End | 127,491,744 |
| Deletion Size | 21,037 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,470,879 | 127,470,901 | CATGCTCATGGAAACCTGGAAAG |
| TUP | 127,470,904 | 127,470,929 | CCAGCCCTTTCCTAATGGGAAGCAGA |
| TUR | 127,470,968 | 127,470,988 | CCCTGTCTAAGCTCACCACTG |
| TDF | 127,491,402 | 127,491,422 | GGCCCTTTCTGTTGAAGGAAC |
| TDP | 127,491,428 | 127,491,451 | TCGCTAGAGATGCTGCGTACTCCA |
| TDR | 127,491,462 | 127,491,484 | TCCCGTGCTTGATGTCATCTTTG |
PCR Primers
| Primer | Sequence |
| SU | GGCTCCTAACAAGACCATGG |
| SD | TGCGTGTGCACTTTGAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12032A-B7 | VGB6 | C57BL/6NTac | |
| 12032A-F12 | VGB6 | C57BL/6NTac | |
| 12032A-A10 | VGB6 | C57BL/6NTac | |
| 12032A-D4 | VGB6 | C57BL/6NTac | |
