| Gene | Kcnab3 |
| Chromosome | 11 |
| Deletion Start | 69,326,685 |
| Deletion End | 69,332,178 |
| Deletion Size | 5,494 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,327,542 | 69,327,561 | TGTGACCCACCTTTGTCCTC |
| TUP | 69,327,567 | 69,327,593 | TATCCAATCTTCTCCCTGTAGCAGCCC |
| TUR | 69,327,600 | 69,327,623 | CACAGATTAAACAGCCCTACTTCC |
| TDF | 69,332,053 | 69,332,071 | GCCGATTCTCAAGGTGCTG |
| TDP | 69,332,075 | 69,332,095 | CAGCTGACGCCGCAAACGGTG |
| TDR | 69,332,096 | 69,332,117 | CCAAGAGCGCATCTATTTCCAC |
PCR Primers
| Primer | Sequence |
| SU | ACCCCACGGTTTTCCTTTCC |
| SD | TCCCAGTGTAGGTTCTTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12034B-C4 | VGB6 | C57BL/6NTac | |
| 12034B-G4 | VGB6 | C57BL/6NTac | |
| 12034B-H4 | VGB6 | C57BL/6NTac | |
| 12034B-G12 | VGB6 | C57BL/6NTac | |
