| Gene | 2510002D24Rik |
| Chromosome | 16 |
| Deletion Start | 18,836,599 |
| Deletion End | 18,839,219 |
| Deletion Size | 2,621 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,837,057 | 18,837,080 | GCGTTAGAGTCATCAGCTTGACAG |
| TUP | 18,837,083 | 18,837,106 | AGCTTGCCTTCACCCAGCCCTTGG |
| TUR | 18,837,132 | 18,837,151 | CTCGTGCTCCAGACACGTTG |
| TDF | 18,838,462 | 18,838,482 | GGCCTACATAGCACCTGACAC |
| TDP | 18,838,485 | 18,838,511 | TGCCAGGATCAGCCCTGTCATAATACA |
| TDR | 18,838,519 | 18,838,536 | GCCACTGCCAACAGACAC |
PCR Primers
| Primer | Sequence |
| SU | TGCCTTCCTCCTCTCCATAC |
| SD | TTGCAGTCCATCTCTCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12035A-C5 | VGB6 | C57BL/6NTac | |
| 12035A-E11 | VGB6 | C57BL/6NTac | |
| 12035A-D6 | VGB6 | C57BL/6NTac | |
| 12035A-A11 | VGB6 | C57BL/6NTac | |
| 12035A-B12 | VGB6 | C57BL/6NTac | |
