| Gene | |
| Chromosome | 5 |
| Deletion Start | 129,716,747 |
| Deletion End | 129,718,169 |
| Deletion Size | 1,423 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 129,717,282 | 129,717,302 | GCACGGGAACAGGATAAACTC |
| TUP | 129,717,308 | 129,717,334 | CCCTTCTTCCCAGGTTGCCATAAGTCA |
| TUR | 129,717,345 | 129,717,363 | GGGTCGGCTCAAATGTCTC |
| TDF | 129,717,974 | 129,717,992 | GGGACATCGTGCTCCTCAG |
| TDP | 129,717,995 | 129,718,018 | CTTTGCCCGTCCCACGATCGAAGC |
| TDR | 129,718,037 | 129,718,060 | GGCCGACTTTGAAGATGATCTCAG |
PCR Primers
| Primer | Sequence |
| SU | GGGTATTGTGGCACCAGC |
| SD | GATGGCTCAGTGGGTACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12037C-E3 | VGB6 | C57BL/6NTac | |
| 12037C-H4 | VGB6 | C57BL/6NTac | |
| 12037B-F3 | VGB6 | C57BL/6NTac | |
