| Gene | Tmem43 |
| Chromosome | 6 |
| Deletion Start | 91,473,890 |
| Deletion End | 91,487,042 |
| Deletion Size | 13,153 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,474,495 | 91,474,513 | TGGGAGAAGCCGGTGAGTC |
| TUP | 91,474,516 | 91,474,538 | CAGTGCCTCTGGTGAAAGCGCCT |
| TUR | 91,474,546 | 91,474,564 | TGGCAGTGCTCCTGAGATG |
| TDF | 91,486,314 | 91,486,335 | CCCTCCCTGTCCATCAGTAAAC |
| TDP | 91,486,337 | 91,486,359 | TGACCTGCTGAACCCTGGGTCCT |
| TDR | 91,486,386 | 91,486,406 | CAGAGGACGGGCTTGTCTATC |
PCR Primers
| Primer | Sequence |
| SU | TTTCCCAGAAATCTTGCAGC |
| SD | AACCAAGAACCAGAAGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12038C-B11 | VGB6 | C57BL/6NTac | |
| 12038C-G9 | VGB6 | C57BL/6NTac | |
| 12038D-B2 | VGB6 | C57BL/6NTac | |
| 12038D-A6 | VGB6 | C57BL/6NTac | |
| 12038D-F3 | VGB6 | C57BL/6NTac | |
