| Gene | Tuba1a |
| Chromosome | 15 |
| Deletion Start | 98,953,450 |
| Deletion End | 98,950,029 |
| Deletion Size | 3,422 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,953,123 | 98,953,143 | GAGCCCGAACTCCAGAAACAC |
| TUP | 98,953,075 | 98,953,104 | AAATGTTTAGTTTGCGTCGGATCCATGGCA |
| TUR | 98,953,019 | 98,953,039 | AACGCCACGGAAGCAAGAGAC |
| TDF | 98,950,757 | 98,950,781 | GGTAGACAATGAGGCCATCTATGAT |
| TDP | 98,932,275 | 98,932,301 | TGTCGTAGAAACCTCGACATTGAGCGC |
| TDR | 98,950,675 | 98,950,700 | TGATGGAAGACACAATTTGACCTATC |
PCR Primers
| Primer | Sequence |
| SU | TCTTTCTTCCCTTTCCACCC |
| SD | TTTCAGCCAAAGCATGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12039A-A2 | VGB6 | C57BL/6NTac | |
| 12039A-C3 | VGB6 | C57BL/6NTac | |
| 12039A-E5 | VGB6 | C57BL/6NTac | |
| 12039A-E9 | VGB6 | C57BL/6NTac | |
| 12039A-F2 | VGB6 | C57BL/6NTac | |
| 12039A-H4 | VGB6 | C57BL/6NTac | |
