| Gene | Commd5 |
| Chromosome | 15 |
| Deletion Start | 76,900,408 |
| Deletion End | 76,901,122 |
| Deletion Size | 715 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,900,426 | 76,900,448 | GCTCCATACTTACACCATCCAAC |
| TUP | 76,900,453 | 76,900,476 | AGTCACAGCGGCCGGGTCAGTTTC |
| TUR | 76,900,554 | 76,900,575 | CCCGACTACGAGTTTCAACAAC |
| TDF | 76,900,889 | 76,900,910 | CCATCTCAACCAGTGCTCAGTC |
| TDP | 76,900,912 | 76,900,933 | CGCTCCCTGCAACCGAGTGTTC |
| TDR | 76,900,950 | 76,900,969 | GGTGTGCCGATCCATCTGTG |
PCR Primers
| Primer | Sequence |
| SU | GCGCTTCAGTTTCTTGGTTC |
| SD | AGAATAAACACGTCCGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 150 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12041A-A7 | VGB6 | C57BL/6NTac | |
| 12041A-A9 | VGB6 | C57BL/6NTac | |
| 12041A-A10 | VGB6 | C57BL/6NTac | |
| 12041A-G8 | VGB6 | C57BL/6NTac | |
| 12041A-F12 | VGB6 | C57BL/6NTac | |
| 12041A-G11 | VGB6 | C57BL/6NTac | |
