| Gene | Snapin |
| Chromosome | 3 |
| Deletion Start | 90,490,971 |
| Deletion End | 90,489,455 |
| Deletion Size | 1,517 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,490,277 | 90,490,298 | GTTGGTTGCCCATTTCCTCTTC |
| TUP | 90,490,251 | 90,490,276 | CCAGAACTGTGCCGGATCAATGAGGA |
| TUR | 90,490,229 | 90,490,247 | CAGATCCAGGGCCACTTTC |
| TDF | 90,489,568 | 90,489,590 | CGACTAAGGCGGTTAAACCACAG |
| TDP | 90,489,545 | 90,489,565 | TGGCCAAGGAAACAGCTCGCA |
| TDR | 90,489,502 | 90,489,523 | GGAGAACCAGGAGGGTAAACTC |
PCR Primers
| Primer | Sequence |
| SU | CAGCCTGGTTTACATAGTTAC |
| SD | TCTACCACACGATGCCTAATTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12045A-D3 | VGB6 | C57BL/6NTac | |
| 12045A-A7 | VGB6 | C57BL/6NTac | |
| 12045B-A4 | VGB6 | C57BL/6NTac | |
| 12045B-C10 | VGB6 | C57BL/6NTac | |
| 12045B-E11 | VGB6 | C57BL/6NTac | |
| 12045B-F5 | VGB6 | C57BL/6NTac | |
| 12045B-E2 | VGB6 | C57BL/6NTac | |
| 12045B-A5 | VGB6 | C57BL/6NTac | |
