| Gene | Mlc1 |
| Chromosome | 15 |
| Deletion Start | 88,977,990 |
| Deletion End | 88,956,956 |
| Deletion Size | 21,035 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,977,746 | 88,977,764 | TGGTCCAGTGTCCCTTCAG |
| TUP | 88,977,723 | 88,977,745 | TGCTGGTCATGCCCTGTAGAGCA |
| TUR | 88,977,703 | 88,977,722 | TCCCAGTCTATCGGGCTTTG |
| TDF | 88,957,268 | 88,957,291 | GGAAGGGATGTGGGAATGTCTAAG |
| TDP | 88,957,246 | 88,957,267 | ATGCTTCGTCCCTGCCCGGGAG |
| TDR | 88,957,218 | 88,957,240 | TGAGAAAGAACGGAGAAGGGAAG |
PCR Primers
| Primer | Sequence |
| SU | GATGAAGGCTGAGTGTGCTG |
| SD | GAGTCCAGCTGTCTTCTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12047B-B5 | VGB6 | C57BL/6NTac | |
| 12047B-F6 | VGB6 | C57BL/6NTac | |
| 12047B-F2 | VGB6 | C57BL/6NTac | |
| 12047C-B9 | VGB6 | C57BL/6NTac | |
| 12047C-D11 | VGB6 | C57BL/6NTac | |
