| Gene | Alg11 |
| Chromosome | 8 |
| Deletion Start | 22,060,730 |
| Deletion End | 22,068,194 |
| Deletion Size | 7,465 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,060,815 | 22,060,839 | GAGGCAGTCCAGATAAAGTCTGTAG |
| TUP | 22,060,843 | 22,060,867 | CGCTTTCTTCGTGCCTCGGTTTACC |
| TUR | 22,060,909 | 22,060,930 | TGGTTTACGAAGCAGCAGGTAG |
| TDF | 22,067,228 | 22,067,247 | GAGCCCATGCACTGGGTATG |
| TDP | 22,067,248 | 22,067,270 | AGCTCACTGGGCAGCATGAGGAC |
| TDR | 22,067,277 | 22,067,296 | TTACACAGGTGCTGGGACTG |
PCR Primers
| Primer | Sequence |
| SU | CTGGAAGATTAGCTCGTCGC |
| SD | ACTACTGCCTTGCCTATATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12049A-A2 | VGB6 | C57BL/6NTac | |
| 12049A-E5 | VGB6 | C57BL/6NTac | |
