| Gene | Tceal8 |
| Chromosome | X |
| Deletion Start | 136,171,019 |
| Deletion End | 136,170,406 |
| Deletion Size | 614 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,170,827 | 136,170,847 | CAGAGCCCAAAGAGGACACTC |
| TUP | 136,170,804 | 136,170,825 | CGCAAGGCATCTGAACCCCGAA |
| TUR | 136,170,764 | 136,170,785 | CCCTAAGCCTTTCCAACTCATC |
| TDF | 136,170,539 | 136,170,564 | GCGGTATTTTCTGTTATGTAGTTTCG |
| TDP | | | TGTTACCAGCATCTCAT |
| TDR | 136,170,433 | 136,170,458 | GCTGTCTAATTGTATATTTCCATGCA |
PCR Primers
| Primer | Sequence |
| SU | CATTTCAGTGCTAGGAGAGG |
| SD | TGCCTCTACAAGCTGGTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 132 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12051A-B1 | VGB6 | C57BL/6NTac | |
| 12051A-D8 | VGB6 | C57BL/6NTac | |
| 12051A-G7 | VGB6 | C57BL/6NTac | |
