| Gene | Adck2 |
| Chromosome | 6 |
| Deletion Start | 39,574,304 |
| Deletion End | 39,587,522 |
| Deletion Size | 13,219 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,574,642 | 39,574,666 | CTTCTTGTACCCTCTCACCTACTTG |
| TUP | 39,574,671 | 39,574,692 | CTGGCCTCTCCACCCTCTGGCT |
| TUR | 39,574,705 | 39,574,726 | GCCTGAGGTCTCTGTAGCTTTG |
| TDF | 39,587,294 | 39,587,311 | GGCTCAGCGATGTGGTTG |
| TDP | 39,587,312 | 39,587,333 | TCCAGGGTACTCAGCTGCGTCC |
| TDR | 39,587,334 | 39,587,355 | ACAGCAGTTCTTCTCCGACCTC |
PCR Primers
| Primer | Sequence |
| SU | TCTCTGCCTGGTTGTCACTG |
| SD | TTTTGTCGTCTTTTACAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 62 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12053A-G11 | VGB6 | C57BL/6NTac | |
| 12053A-C8 | VGB6 | C57BL/6NTac | |
| 12053A-G1 | VGB6 | C57BL/6NTac | |
| 12053A-G2 | VGB6 | C57BL/6NTac | |
| 12053A-B3 | VGB6 | C57BL/6NTac | |
