| Gene | Lyg1 |
| Chromosome | 1 |
| Deletion Start | 37,952,779 |
| Deletion End | 37,946,857 |
| Deletion Size | 5,923 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,951,905 | 37,951,926 | AGCCAAGGACAATGGCACAATC |
| TUP | 37,951,876 | 37,951,903 | ACTGCCATATTAAGGGCATTTGTCAGCA |
| TUR | 37,951,855 | 37,951,875 | GCACACTTCTGTCTCCATGAC |
| TDF | 37,947,383 | 37,947,406 | GACGAAGAAAGGAGAAGAATGGAG |
| TDP | 37,947,356 | 37,947,382 | TGGAGATGGAAGACGATCCCATACCAC |
| TDR | 37,947,309 | 37,947,329 | CCTAGTGGGAAAGAGGCAGAG |
PCR Primers
| Primer | Sequence |
| SU | CCCAGCATCTCTGTCTTTCC |
| SD | TAGAGAGCTCGGTGGGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12057A-G4 | VGB6 | C57BL/6NTac | |
| 12057B-D5 | VGB6 | C57BL/6NTac | |
