12058   Bex6     Status: ES cell colonies screened / QC positives

GeneBex6
Chromosome16
Deletion Start32,186,379
Deletion End32,186,798
Deletion Size420
Genome Build38
MGI IDMGI:3588247
NCBI Gene ID328660
BAC ID457P17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF32,186,43932,186,460GCCTCCAGACACAGTGAAGAAG
TUP32,186,46432,186,490CCCACCTTCTGGAAGAGGTAGAGACCA
TUR32,186,50832,186,529GTCGCCTGACTTTCCTTTTGAC
TDF32,186,59632,186,618CTGGGAGATTTGTAGGGCAGGAG
TDP32,186,62032,186,647CAGAAGCCAAGAGAAAGAGTCAGGAGCA
TDR32,186,66332,186,682CCGGTGTGAGGAACCACTTG

PCR Primers

PrimerSequence
SUAACACTGAACATAGCCACTAG
SDATGGCCACTGAGTTACGAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR91 bp++-
TDFTDR87 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12058A-B5VGB6C57BL/6NTac
12058A-D5VGB6C57BL/6NTac
12058A-E7VGB6C57BL/6NTac
12058A-F1VGB6C57BL/6NTac
12058A-G1VGB6C57BL/6NTac
12058A-H11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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