| Gene | Sh3bp1 |
| Chromosome | 15 |
| Deletion Start | 78,899,906 |
| Deletion End | 78,912,047 |
| Deletion Size | 12,142 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,900,449 | 78,900,470 | TCGTAGGCTCAGGTAGAGACAC |
| TUP | 78,900,471 | 78,900,492 | CCCTTGGTCTCCTCGGTTCCCA |
| TUR | 78,900,502 | 78,900,522 | CCCAAACTTCCGCTCCCATAG |
| TDF | 78,911,140 | 78,911,159 | GGAGGACCACTAGGGAACAC |
| TDP | 78,911,161 | 78,911,183 | TCAACTGCAGAGGAGGACCACCA |
| TDR | 78,911,215 | 78,911,236 | GGTGGGATGGAGTTTCACTTTC |
PCR Primers
| Primer | Sequence |
| SU | CTCAGAGAGACAGACCGAC |
| SD | TCAGAACACAGACTGGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12060A-B9 | VGB6 | C57BL/6NTac | |
| 12060A-E3 | VGB6 | C57BL/6NTac | |
| 12060A-F5 | VGB6 | C57BL/6NTac | |
| 12060A-F6 | VGB6 | C57BL/6NTac | |
